Team:HokkaidoU Japan/Test

From 2010.igem.org

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=HokkaidoU_Japan Team Wiki for iGEM 2010=
 
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[[Image:HokkaidoU_Japan_team.png|thumb|200px|left|Team]]
 
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== Dr. E.coli : The smallest protein injector in the world ==
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==The team==
 
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There are several transfection methods to introduce nucleic acid into cells, however methods to introduce “protein” directly into cells are complicated.
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Unlike introducing DNA, which changes cell genetic makeup permenantly, proteins can act at pinpoint acuracy. And afterwards be degraded leaving no permanent information in the target cell,
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==The project==
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Our mission was to find an easy way to introduce desired proteins in the cell. Speficaly into eucaryotic cell. Order to do this we selected Type 3 Secreation System. Find in Salmonella and EPEc. These bacteria are pathogenic and working with them is problematic. To make things easier and safer we ordered DNA fragment which contains T3SS. And introduce it into E.coli.
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We also needed protein to secreat. We done it by ataching secreation signal to desired protein. Our choice was GFP. Also added NLS to make it go to nucleus.
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Currently we will test tjis system on liver cancer cells to see if GFP will localize into nucleus.
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--[[User:Yuichi|Yuichi]] 08:06, 28 July 2010 (UTC)
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Revision as of 01:49, 8 October 2010


Dr. E.coli : The smallest protein injector in the world

There are several transfection methods to introduce nucleic acid into cells, however methods to introduce “protein” directly into cells are complicated. Unlike introducing DNA, which changes cell genetic makeup permenantly, proteins can act at pinpoint acuracy. And afterwards be degraded leaving no permanent information in the target cell, Our mission was to find an easy way to introduce desired proteins in the cell. Speficaly into eucaryotic cell. Order to do this we selected Type 3 Secreation System. Find in Salmonella and EPEc. These bacteria are pathogenic and working with them is problematic. To make things easier and safer we ordered DNA fragment which contains T3SS. And introduce it into E.coli. We also needed protein to secreat. We done it by ataching secreation signal to desired protein. Our choice was GFP. Also added NLS to make it go to nucleus.

Currently we will test tjis system on liver cancer cells to see if GFP will localize into nucleus.
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Test"