Team:HokkaidoU Japan/ProjectTest

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Template:HokkaidoU_Japan}}
{{Template:HokkaidoU_Japan}}
-
=Project Abstract=
+
=PCR based assembly protocol=
-
<p>
+
In standard protocol, DNA fragment is amplified via transformation and miniprep. But this takes too long time you know. Moreover, minipreped plasmid is impure in most case.
-
Our project is on Type III Secretion Apparatus which is one of the most amazing biological devices. It can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. This apparatus which looks like a syringe is an organelle of pathogenic gram-negative
+
-
bacterium such as Salmonella and Yersinia. We are aiming at making this device available for ''E. coli''. Because it will not involve usage of pathogenic strains, it will be safer to use. To transfer T3SS functionally from Salmonella to ''E. coli'' it is essential to integrate at least 40kb of DNA fragment coding more than 20 proteins. So we will make suggestions about how to optimize ''E. coli'' transformation method for large size DNA fragments. Also we will show how to construct protein for secretion and how to measure if it is really secreted using GFP.
+
-
</p>
+
-
 
+
-
 
+
-
==Construction of GFP injector by ''E. coli''==
+
-
[[Image:HokkaidoU Japan Abstract tr.png|center]]
+
-
 
+
-
 
+
-
 
+
-
==Methods for Infection Analysis==
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==Results==
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==Discussion==
+
-
 
+
-
 
+
-
 
+
-
==Acknowledgements==
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==References==
+

Revision as of 11:12, 27 October 2010

PCR based assembly protocol

In standard protocol, DNA fragment is amplified via transformation and miniprep. But this takes too long time you know. Moreover, minipreped plasmid is impure in most case.
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/ProjectTest"