Team:HokkaidoU Japan/Notebook/September8
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- | = | + | =Colony PCR= |
- | PS-R, EX- | + | Checked if primers PS-R, EX-F were good to use for colony PCR and also checked if insert was realy in plasmids |
- | * | + | * September 6th transformation (digestion was done with H buffer×2 and M buffer×2, 2 version) |
* 1-3A | * 1-3A | ||
- | * | + | * Retried September 6th transformation |
* pUC119 | * pUC119 | ||
Revision as of 17:57, 27 September 2010
Colony PCR
Checked if primers PS-R, EX-F were good to use for colony PCR and also checked if insert was realy in plasmids
- September 6th transformation (digestion was done with H buffer×2 and M buffer×2, 2 version)
- 1-3A
- Retried September 6th transformation
- pUC119
pSB1C3の確認
1-3A(RFP reporter)由来のpSB1C3を使っているため,RFP発現がこれに由来している可能性が無くない
- H buffer処理 2 uL + Mighty Mix 2 uL + T4 ligase 0.5 uL → Ligation → Transformation
- M buffer処理 2 uL + Mighty Mix 2 uL + T4 ligase 0.5 uL → Ligation → Transformation
- H buffer処理 2 uL → Transformation
- H buffer処理 2 uL → Transformation
GFPのPCR
- GFP reporter BBa_I13522 pSB1A2 2-8A 937bp
- GFP protein BBa_E0840 pSB1A2 1-12O 878bp