Team:HokkaidoU Japan/Notebook/September7

From 2010.igem.org

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(→エタ沈)
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In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done.
In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done.
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==コンピテントセルのPCR==
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==Colony PCR of competent cells==
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先日のコロニーPCRで謎なバンドがすべてにみられたので，大腸菌ゲノム由来のものである可能性をみるため，コンピテントセルをPCRにかけたところ，バンドはみられなかった
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* すべてにみられたバンドはベクター由来か
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Previous colony PCR produced strange bands so checked if there were from DNA of competent cell
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* Colony PCR didn`t produce any bands so those bands had to be an artifact from plasmid used for transformation

Revision as of 17:53, 27 September 2010

Notebook

Checked concentration of DNA used yestrday for ligation

  • 4 ng/uL RFP digested using either H buffer and M buffer
  • pSB1C3 band was very week

Ethanol percipication

In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done.

Colony PCR of competent cells

Previous colony PCR produced strange bands so checked if there were from DNA of competent cell

  • Colony PCR didn`t produce any bands so those bands had to be an artifact from plasmid used for transformation
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