Team:HokkaidoU Japan/Notebook/September29

From 2010.igem.org

(Difference between revisions)
Line 6: Line 6:
*electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.
*electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.
 +
Line 79: Line 80:
|-
|-
|}
|}
 +
 +
[[Image:HokkaidoU Japan 20100929b.JPG‎|200px|left|thumb|Electrophoresis of colony PCR sample]]
*erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
*erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
 +
*
*

Revision as of 16:21, 27 October 2010

Electrophoresis of Yesterday Samples

Electrophoresis of concentrated GFP-double terminator(T3SS signal wasn't seen)
  • electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.

















Colony PCR of Arabinose Promoter + RBS + GFP + double terminator

  • PCRed 5 colony.


PCR mix

Reagent Amount
Quick Taq 25 uL
EX-F 0.5 uL
PS-R 0.5 uL
Total 26 uL
  • PCRed according to the table below.98C and 68C for 35 cycles.


temp time
94C 2 min
94C 30 sec
68C 90 sec
4C hold
File:HokkaidoU Japan 20100929b.JPG
Electrophoresis of colony PCR sample
  • erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.