Team:HokkaidoU Japan/Notebook/September29

From 2010.igem.org

(Difference between revisions)
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= Colony PCR of Arabinose Promoter + RBS + GFP + double terminator =
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*PCRed 5 colony.
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 +
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PCR mix
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{|style="text-align: center" class="protocol"
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!Reagent
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!Amount
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|-
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|Quick Taq
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|25 uL
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|-
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|EX-F
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|0.5 uL
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|-
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|PS-R
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|0.5 uL
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|-
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|style="border-top:1px solid #996"|'''Total'''
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|style="border-top:1px solid #996"|'''26 uL'''
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|}
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*PCRed according to the table below.98C and 68C for 35 cycles.
<br>
<br>
-
= Colony PCR of Arabinose Promoter + RBS + GFP + double terminator =
+
{|style="text-align: center" class="protocol"
 +
!temp
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!time
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|-
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|94C
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|2 min
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|-
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|94C
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|30 sec
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|-
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|68C
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|90 sec
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|-
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|4C
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|hold
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|-
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|}
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*erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
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*

Revision as of 16:12, 27 October 2010

Notebook

Electrophoresis of Yesterday Samples

Electrophoresis of concentrated GFP-double terminator(T3SS signal wasn't seen)
  • electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.
















Colony PCR of Arabinose Promoter + RBS + GFP + double terminator

  • PCRed 5 colony.


PCR mix

Reagent Amount
Quick Taq 25 uL
EX-F 0.5 uL
PS-R 0.5 uL
Total 26 uL
  • PCRed according to the table below.98C and 68C for 35 cycles.


temp time
94C 2 min
94C 30 sec
68C 90 sec
4C hold
  • erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
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