Team:HokkaidoU Japan/Notebook/September28

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Template:HokkaidoU_Japan}}
{{Template:HokkaidoU_Japan}}
<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September27|September 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September29|September 29]]</div></div>
<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September27|September 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September29|September 29]]</div></div>
 +
 +
== plasmid & GFP-double terminator's Ligation & Transformation ==
 +
#先日凍結した2つのサンプルが乾燥して少なくなっていたので、2 uLのTEを加えて懸濁した。
 +
#2つのサンプルを混合した。
 +
#added 5 uL Mighty mix.
 +
#incubated at 16C for 30min.
 +
#added the sample to 100 uL competent cell.
 +
#incubated at 0C for 30min.
 +
#heatshocked at 42C for 60sec.
 +
#incubated at 0C for 5min.

Revision as of 13:16, 28 September 2010

plasmid & GFP-double terminator's Ligation & Transformation

  1. 先日凍結した2つのサンプルが乾燥して少なくなっていたので、2 uLのTEを加えて懸濁した。
  2. 2つのサンプルを混合した。
  3. added 5 uL Mighty mix.
  4. incubated at 16C for 30min.
  5. added the sample to 100 uL competent cell.
  6. incubated at 0C for 30min.
  7. heatshocked at 42C for 60sec.
  8. incubated at 0C for 5min.