Team:HokkaidoU Japan/Notebook/September28
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<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September27|September 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September29|September 29]]</div></div> | <div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September27|September 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September29|September 29]]</div></div> | ||
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+ | == plasmid & GFP-double terminator's Ligation & Transformation == | ||
+ | #先日凍結した2つのサンプルが乾燥して少なくなっていたので、2 uLのTEを加えて懸濁した。 | ||
+ | #2つのサンプルを混合した。 | ||
+ | #added 5 uL Mighty mix. | ||
+ | #incubated at 16C for 30min. | ||
+ | #added the sample to 100 uL competent cell. | ||
+ | #incubated at 0C for 30min. | ||
+ | #heatshocked at 42C for 60sec. | ||
+ | #incubated at 0C for 5min. |
Revision as of 13:16, 28 September 2010
plasmid & GFP-double terminator's Ligation & Transformation
- 先日凍結した2つのサンプルが乾燥して少なくなっていたので、2 uLのTEを加えて懸濁した。
- 2つのサンプルを混合した。
- added 5 uL Mighty mix.
- incubated at 16C for 30min.
- added the sample to 100 uL competent cell.
- incubated at 0C for 30min.
- heatshocked at 42C for 60sec.
- incubated at 0C for 5min.