Team:HokkaidoU Japan/Notebook/September16

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(Digestion of GFP and Double Terminator)
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*digestion of GFP(1-14K) and double terminator(1-23L)
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== Digestion of GFP and Double Terminator ==
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Parts Information
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GFP : BBa_E0040 1-14K 720bp pSB1A3
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double terminator : BBa_B0015 1-23L 129bp pSB1AK3
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pSB1A3
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1-14K and pSB1A3 had purified with mycrocon, and 1-23L had extracted from a gel before.
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Method
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1)electrophoresed 1μl of 1-14K and 1-23L added 0.5μl 6×sample buffer to estimate concerntration of each solution.
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2)estimated concerntration from photo of electrophoresys.But I forgot to electrophorese pSB1A3 solution with the other samples,so pSB1A3 solution was done by other person.
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Latest revision as of 17:35, 19 September 2010