araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

• Used TSUDA marker
• Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification

• Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion

→Estimated concentration to be 54 ng/uL

• This time band location is good
• Accidentally excised a part of other band resulting small contamination

GFP(1-12O)ゲル抽精製済の濃度測定

• TSUDA I 2 uL, DNA 0.5 uL

→120 ng/uLと推定

• 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．