Team:HokkaidoU Japan/Notebook/September13

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Notebook

araC promoter purification

HokkaidoU Japan 20100913a.jpg

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

ゲル抽したものを電気泳動

HokkaidoU Japan 20100913b.jpg
  • TSUDA I 2 uL, ゲル抽したものを0.5 uL泳動

→54 ng/uLと推定

  • 今度の位置はちゃんとしている
  • ゲル抽時に別のバンドもとれてしまったみたい

GFP(1-12O)ゲル抽精製済の濃度測定

HokkaidoU Japan 20100913c.jpg
  • TSUDA I 2 uL, DNA 0.5 uL

→120 ng/uLと推定

  • 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
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