Team:HokkaidoU Japan/Notebook/September13

From 2010.igem.org

(Difference between revisions)
(Electrophoresed after Team:HokkaidoU_Japan/Protocolsgel extraction)
(Electrophoresed after gel extraction)
Line 14: Line 14:
[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|Electrophoresis after purification]]
[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|Electrophoresis after purification]]
-
* Electrophoresed TSUDA I[http://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion
+
* Electrophoresed [http://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion
→Estimated concentration to be 54 ng/uL
→Estimated concentration to be 54 ng/uL
* This time band location is good
* This time band location is good

Revision as of 11:34, 16 October 2010

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification
  • Electrophoresed TSUDA I 2 uL and 0.5 uL of purified solutuion

→Estimated concentration to be 54 ng/uL

  • This time band location is good
  • Accidentally excised a part of other band resulting small contamination

Gel purification of GFP(1-12O) and concentration check

Electrophoresis for concentration check

→Estimated concentration to be 120 ng/uL

  • Part length is 878 + 220 bp = 947 bp
  • That slightly above mark so OK.