Team:HokkaidoU Japan/Notebook/September13
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- | =Gel purification of GFP(1-12O) and concentration check= | + | =Gel purification of GFP([[Team:HokkaidoU_Japan/Material_And_Methods#Materials_And_Methods|1-12O]]) and concentration check= |
[[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|Electrophoresis for concentration check]] | [[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|Electrophoresis for concentration check]] |
Revision as of 07:38, 1 October 2010
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
Electrophoresed after gel extraction
- Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion
→Estimated concentration to be 54 ng/uL
- This time band location is good
- Accidentally excised a part of other band resulting small contamination
Gel purification of GFP(1-12O) and concentration check
- TSUDA I 2 uL, DNA 0.5 uL
→Estimated concentration to be 120 ng/uL
- Part length is 878 + 220 bp = 947 bp
- That slightly above mark so OK.