Team:HokkaidoU Japan/Notebook/September13
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=Electrophoresed after gel extraction= | =Electrophoresed after gel extraction= | ||
- | [[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|]] | + | [[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|Electrophoresis after purification]] |
* Electrophoresed TSUDA I[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion | * Electrophoresed TSUDA I[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion |
Revision as of 06:22, 28 September 2010
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
Electrophoresed after gel extraction
- Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion
→Estimated concentration to be 54 ng/uL
- This time band location is good
- Accidentally excised a part of other band resulting small contamination
GFP(1-12O)ゲル抽精製済の濃度測定
- TSUDA I 2 uL, DNA 0.5 uL
→120 ng/uLと推定
- 878 + 220 bpが947 bpのマーカーのちょい上に来ている.OK.