Team:HokkaidoU Japan/Notebook/September13

From 2010.igem.org

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(→araC promoter purification)
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=ゲル抽したものを電気泳動=
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=Electrophoresed after gel extraction=
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[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|]]
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* TSUDA I 2 uL, ゲル抽したものを0.5 uL泳動
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→54 ng/uLと推定
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* Electrophoresed TSUDA I[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion
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* 今度の位置はちゃんとしている
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→Estimated concentration to be 54 ng/uL
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* ゲル抽時に別のバンドもとれてしまったみたい
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* This time band location is good
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* Accidentally excised a part of other band resulting small contamination
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Revision as of 06:17, 28 September 2010

Notebook

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

Electrophoresed after gel extraction

HokkaidoU Japan 20100913b.jpg
  • Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion

→Estimated concentration to be 54 ng/uL

  • This time band location is good
  • Accidentally excised a part of other band resulting small contamination

GFP(1-12O)ゲル抽精製済の濃度測定

HokkaidoU Japan 20100913c.jpg
  • TSUDA I 2 uL, DNA 0.5 uL

→120 ng/uLと推定

  • 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
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