Team:HokkaidoU Japan/Notebook/September13

From 2010.igem.org

(Difference between revisions)
(→araCプロモーターのゲル抽精製)
(→araC promoter purification)
Line 3: Line 3:
=araC promoter purification=
=araC promoter purification=
-
[[Image:HokkaidoU Japan 20100913a.jpg‎|200px|right|thumb|]]
+
[[Image:HokkaidoU Japan 20100913a.jpg‎|200px|right|thumb|Electrophoresed for gel extraction]]
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

Revision as of 06:12, 28 September 2010

Notebook

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

ゲル抽したものを電気泳動

HokkaidoU Japan 20100913b.jpg
  • TSUDA I 2 uL, ゲル抽したものを0.5 uL泳動

→54 ng/uLと推定

  • 今度の位置はちゃんとしている
  • ゲル抽時に別のバンドもとれてしまったみたい

GFP(1-12O)ゲル抽精製済の濃度測定

HokkaidoU Japan 20100913c.jpg
  • TSUDA I 2 uL, DNA 0.5 uL

→120 ng/uLと推定

  • 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/September13"