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Team:HokkaidoU Japan/Notebook/September13
From 2010.igem.org
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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September10|September 10]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September14|September 14]]</div></div> | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September10|September 10]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September14|September 14]]</div></div> | ||
| - | = | + | =araC promoter purification= |
| + | |||
[[Image:HokkaidoU Japan 20100913a.jpg|200px|right|thumb|]] | [[Image:HokkaidoU Japan 20100913a.jpg|200px|right|thumb|]] | ||
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| - | * | + | Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway. |
| - | * | + | * Used [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA] marker |
| + | * Part length is 1259 bp | ||
<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
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=ゲル抽したものを電気泳動= | =ゲル抽したものを電気泳動= | ||
[[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|]] | [[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|]] | ||
Revision as of 06:12, 28 September 2010
since 13 Jul 2010
since 1 Aug 2010
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
ゲル抽したものを電気泳動
- TSUDA I 2 uL, ゲル抽したものを0.5 uL泳動
→54 ng/uLと推定
- 今度の位置はちゃんとしている
- ゲル抽時に別のバンドもとれてしまったみたい
GFP(1-12O)ゲル抽精製済の濃度測定
- TSUDA I 2 uL, DNA 0.5 uL
→120 ng/uLと推定
- 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
