Team:HokkaidoU Japan/Notebook/September13

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=Electrophoresed after gel extraction=
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=Electrophoresed after [[Team:HokkaidoU_Japan/Protocols|gel extraction]]=
[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|Electrophoresis after purification]]
[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|Electrophoresis after purification]]
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* Electrophoresed TSUDA I[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solutuion
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* Electrophoresed [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solution
→Estimated concentration to be 54 ng/uL
→Estimated concentration to be 54 ng/uL
* This time band location is good
* This time band location is good
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=GFP(1-12O)ゲル抽精製済の濃度測定=
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=Gel purification of GFP([[Team:HokkaidoU_Japan/Material_And_Methods#Materials_And_Methods|1-12O]]) and concentration check=
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|]]
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* TSUDA I 2 uL, DNA 0.5 uL
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|Electrophoresis for concentration check]]
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→120 ng/uLと推定
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* 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
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* [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL
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→Estimated concentration to be 120 ng/uL
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* Part length is 878 + 220 bp = 947 bp
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* That slightly above mark so OK．

Latest revision as of 08:15, 27 October 2010

Notebook

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification
  • Electrophoresed TSUDA I 2 uL and 0.5 uL of purified solution

→Estimated concentration to be 54 ng/uL

  • This time band location is good
  • Accidentally excised a part of other band resulting small contamination

Gel purification of GFP(1-12O) and concentration check

Electrophoresis for concentration check

→Estimated concentration to be 120 ng/uL

  • Part length is 878 + 220 bp = 947 bp
  • That slightly above mark so OK．
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