Team:HokkaidoU Japan/Notebook/September13
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=araC promoter purification= | =araC promoter purification= | ||
- | [[Image:HokkaidoU Japan 20100913a.jpg|200px|right|thumb|]] | + | [[Image:HokkaidoU Japan 20100913a.jpg|200px|right|thumb|Electrophoresed for gel extraction]] |
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway. | Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway. | ||
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<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
- | = | + | =Electrophoresed after [[Team:HokkaidoU_Japan/Protocols|gel extraction]]= |
- | [[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|]] | + | |
- | * TSUDA I 2 uL | + | [[Image:HokkaidoU Japan 20100913b.jpg|200px|right|thumb|Electrophoresis after purification]] |
- | + | ||
- | * | + | * Electrophoresed [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solution |
- | * | + | →Estimated concentration to be 54 ng/uL |
+ | * This time band location is good | ||
+ | * Accidentally excised a part of other band resulting small contamination | ||
<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
- | =GFP(1-12O) | + | =Gel purification of GFP([[Team:HokkaidoU_Japan/Material_And_Methods#Materials_And_Methods|1-12O]]) and concentration check= |
- | [[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|]] | + | |
- | * TSUDA I 2 uL, DNA 0.5 uL | + | [[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|Electrophoresis for concentration check]] |
- | + | ||
- | * 878 + 220 | + | * [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL |
+ | →Estimated concentration to be 120 ng/uL | ||
+ | * Part length is 878 + 220 bp = 947 bp | ||
+ | * That slightly above mark so OK. |
Latest revision as of 08:15, 27 October 2010
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
Electrophoresed after gel extraction
- Electrophoresed TSUDA I 2 uL and 0.5 uL of purified solution
→Estimated concentration to be 54 ng/uL
- This time band location is good
- Accidentally excised a part of other band resulting small contamination
Gel purification of GFP(1-12O) and concentration check
- TSUDA I 2 uL, DNA 0.5 uL
→Estimated concentration to be 120 ng/uL
- Part length is 878 + 220 bp = 947 bp
- That slightly above mark so OK.