Team:HokkaidoU Japan/Notebook/September13

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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September10|September 10]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September14|September 14]]</div></div>
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=araCプロモーターのゲル抽精製=
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[[Image:HokkaidoU Japan 20100913a.jpg‎|200px|right|thumb|]]
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=araC promoter purification=
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上のバンドはマーカーと比較するとやや小さいが，量が多すぎて流れたものと考え，これをゲル抽
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* TSUDAの一番上は1250 bp
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[[Image:HokkaidoU Japan 20100913a.jpg‎|200px|right|thumb|Electrophoresed for gel extraction]]
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* 目的のバンドは1259 bp
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Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
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* Used [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA] marker
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* Part length is 1259 bp
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=ゲル抽したものを電気泳動=
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[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|]]
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=Electrophoresed after [[Team:HokkaidoU_Japan/Protocols|gel extraction]]=
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* TSUDA I 2 uL, ゲル抽したものを0.5 uL泳動
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→54 ng/uLと推定
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[[Image:HokkaidoU Japan 20100913b.jpg‎|200px|right|thumb|Electrophoresis after purification]]
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* 今度の位置はちゃんとしている
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* ゲル抽時に別のバンドもとれてしまったみたい
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* Electrophoresed [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL and 0.5 uL of purified solution
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→Estimated concentration to be 54 ng/uL
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* This time band location is good
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* Accidentally excised a part of other band resulting small contamination
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=GFP(1-12O)ゲル抽精製済の濃度測定=
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=Gel purification of GFP([[Team:HokkaidoU_Japan/Material_And_Methods#Materials_And_Methods|1-12O]]) and concentration check=
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|]]
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* TSUDA I 2 uL, DNA 0.5 uL
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|Electrophoresis for concentration check]]
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→120 ng/uLと推定
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* 878 + 220 bpが947 bpのマーカーのちょい上に来ている．OK．
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* [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL
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→Estimated concentration to be 120 ng/uL
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* Part length is 878 + 220 bp = 947 bp
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* That slightly above mark so OK．

Latest revision as of 08:15, 27 October 2010

Notebook

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification
  • Electrophoresed TSUDA I 2 uL and 0.5 uL of purified solution

→Estimated concentration to be 54 ng/uL

  • This time band location is good
  • Accidentally excised a part of other band resulting small contamination

Gel purification of GFP(1-12O) and concentration check

Electrophoresis for concentration check

→Estimated concentration to be 120 ng/uL

  • Part length is 878 + 220 bp = 947 bp
  • That slightly above mark so OK．
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