Revision as of 17:13, 27 September 2010

PCR of 1-5A（RFP reporter）

Because part lenght was 1069bp plus primer added 300bp to that, the extension time was 90 sec
PCR product was purified via Microcon YM-10

Electrophoresis of purified PCR product

Electrophoresed pUC119，pSB1C3，1-5A to check concentration （1 uL of each）

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 {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August31|August 31]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September2|September 2]]</div></div>
-=1-5A（RFP reporter）のPCR=
+=PCR of 1-5A（RFP reporter）=
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-* パーツは1069bp，primerは300bpのため，余裕を持ってExtensionは90秒で行った
+* Because part lenght was 1069bp plus primer added 300bp to that, the extension time was 90 sec
-* 得られたPCR solutionをMicrocon YM-10でろ過した
+* PCR product was purified via Microcon YM-10
-===電気泳動===
-[[Image:HokkaidoU Japan 20100901a.JPG‎|200px|right|thumb|]]
+===Electrophoresis===
-pUC119，pSB1C3，1-5Aの濃度を測定するため，電気泳動した（各1 uL使用）
-* 2: λ/''Hin''d III（2 uL)
+[[Image:HokkaidoU Japan 20100901a.JPG‎|200px|right|thumb|Electrophoresis of purified PCR product]]
+Electrophoresed pUC119，pSB1C3，1-5A to check concentration （1 uL of each）
+* 2: [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png Lambda/''Hin''d III]（2 uL)
 * 3: pUC119 (3000bp): 10 ng/uL
 * 4: pSB1C3 (2000bp): 20 ng/uL
 * 5: 1-5A (1000bp): 80 ng/uL