Team:HokkaidoU Japan/Notebook/October3

From 2010.igem.org

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Revision as of 06:33, 4 October 2010

Notebook

Miniprep

HokkaidoU Japan 20101003a.JPG

コロニー8, 10, 11, 12, 13, 14, 15, 16からミニプレキットでプラスミドを精製した
->うち1 uLを泳動して確認

  • Insertはおよそ1800 bp, Vectorはおよそ2500 b
  • コントロールの16番以外，それらしいバンドがある
  • 2 ug/uLくらいのDNA量と推定

Insertの確認

HokkaidoU Japan 20101003b.JPG

Miniprepで得たプラスミドをNot Iで処理すれば，約1800 bpのInsertが得られるはずである

well DNA RE
1 λ/Hind III (2 uL)
2 pSB1T3 (1 uL)
3 No.8 Not I
4 No.8 EcoR I
5 No.8
6 No.10 Not I
7 No.10 EcoR I
8 No.10
9 No.11 Not I
10 No.11 EcoR I
11 No.11
12 No.14 Not I
13 No.14 EcoR I
14 No.14
15 No.16 Not I
16 No.16 EcoR I
17 No.16
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