Team:HokkaidoU Japan/Notebook/October2

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(New page: == Colony PCR == * Performed Colonie PCR for 3 colonies which were incubated over night * Colony numbers were: 1, 2 and 3 * Results showed no insertion ** but when result came, had alread...)
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== Colony PCR ==
== Colony PCR ==
* Performed Colonie PCR for 3 colonies which were incubated over night
* Performed Colonie PCR for 3 colonies which were incubated over night

Revision as of 05:19, 2 October 2010

Notebook

Colony PCR

  • Performed Colonie PCR for 3 colonies which were incubated over night
  • Colony numbers were: 1, 2 and 3
  • Results showed no insertion
    • but when result came, had already done miniprep and prepared Sequencing Master Mix

Miniprep

  • Used Qiagen kit
  • Melted in 50 uL TE instead of H2O

Preparation for Sequencing

  • Mixed as shown in the table below


5x Sequencing Buffer 1.5uL 24.75 uL Ready Reaction Premix 1 uL 16.5 H2O 5 uL 80 uL total 7.5/sample

Reagent Amount Amount for 16.5
5x Sequencing Buffer 1.5uL 24.75 uL
Ready Reaction Premix 1 uL 16.5
H2O 5 uL 80 uL
Total 7.5/sample 121.25
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