Team:HokkaidoU Japan/Notebook/August31

From 2010.igem.org

Gel extraction of pUC119, Ligation and Transformation

  • Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL
  • Divided among 3 lanes
  • Gel extraction (gel weight: 220 mg)
  • Final volume after extraction was50 uL(500 ng/uL)

Ligation

Empty I M
DNA 2 uL 2 uL 2 uL
ligation solution I 2 uL
Mighty Mix 2 uL
T4 ligase 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
Transformation