Revision as of 17:08, 27 September 2010

Gel extraction of pUC119, Ligation and Transformation

→Transformation

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-=pUC119のゲル抽＆Ligation&Transformation=
+=Gel extraction of pUC119, Ligation and Transformation=
-* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
+* Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL
-* ゲル抽出(220 mg)
+* Divided among 3 lanes
-* 50 uL（500 ng/uL）になったとする
+* Gel extraction (gel weight: 220 mg)
+* Final volume after extraction was50 uL（500 ng/uL）
 ===Ligation===
 {|style="text-align:center;" class="protocol"
 |-
 |style="border-right:1px solid #996; border-bottom:1px solid #996; width:100px"|
-|style="border-bottom:1px solid #996;"|なし
+|style="border-bottom:1px solid #996;"|Empty
 |style="border-bottom:1px solid #996;"|I
 |style="border-bottom:1px solid #996;"|M