Team:HokkaidoU Japan/Notebook/August31

From 2010.igem.org

(Difference between revisions)
(→Ligation)
(→Ligation)
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{|style="text-align:center;" class="protocol"
{|style="text-align:center;" class="protocol"
|-
|-
-
|style="border-right:1px solid #000; border-bottom:1px solid #000; width:100px"|
+
|style="border-right:1px solid #996; border-bottom:1px solid #996; width:100px"|
-
|style="border-bottom:1px solid #000;"|なし
+
|style="border-bottom:1px solid #996;"|なし
-
|style="border-bottom:1px solid #000;"|I
+
|style="border-bottom:1px solid #996;"|I
-
|style="border-bottom:1px solid #000;"|M
+
|style="border-bottom:1px solid #996;"|M
|-
|-
-
|style="border-right:1px solid #000;"|DNA
+
|style="border-right:1px solid #996;"|DNA
|2 uL
|2 uL
|2 uL
|2 uL
|2 uL
|2 uL
|-
|-
-
|style="border-right:1px solid #000;"|ligation solution I
+
|style="border-right:1px solid #996;"|ligation solution I
|－
|－
|2 uL
|2 uL
|－
|－
|-
|-
-
|style="border-right:1px solid #000;"|Mighty Mix
+
|style="border-right:1px solid #996;"|Mighty Mix
|－
|－
|－
|－
|2 uL
|2 uL
|-
|-
-
|style="border-right:1px solid #000;"|T4 ligase
+
|style="border-right:1px solid #996;"|T4 ligase
|－
|－
|0.4 uL
|0.4 uL
|0.4 uL
|0.4 uL
|-
|-
-
|style="border-right:1px solid #000; border-top:1px solid #000;"|Total
+
|style="border-right:1px solid #996; border-top:1px solid #996;"|Total
-
|style="border-top:1px solid #000;"|2 uL
+
|style="border-top:1px solid #996;"|2 uL
-
|style="border-top:1px solid #000;"|4.4 uL
+
|style="border-top:1px solid #996;"|4.4 uL
-
|style="border-top:1px solid #000;"|4.4 uL
+
|style="border-top:1px solid #996;"|4.4 uL
|}
|}
→Transformation
→Transformation

Revision as of 07:06, 20 September 2010

Notebook

pUC119のゲル抽＆Ligation&Transformation

  • pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
  • ゲル抽出(220 mg)
  • 50 uL（500 ng/uL）になったとする

Ligation

なし I M
DNA 2 uL 2 uL 2 uL
ligation solution I － 2 uL －
Mighty Mix － － 2 uL
T4 ligase － 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
→Transformation
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