Team:HokkaidoU Japan/Notebook/August31

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=pUC119のゲル抽＆Ligation&Transformation=
=pUC119のゲル抽＆Ligation&Transformation=
* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した

Revision as of 15:01, 18 September 2010

Notebook

pUC119のゲル抽＆Ligation&Transformation

  • pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
  • ゲル抽出(220 mg)
  • 50 uL（500 ng/uL）になったとする

Ligation

なし I M
DNA 2 uL 2 uL 2 uL
ligation solution I － 2 uL －
Mighty Mix － － 2 uL
T4 ligase － 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
→Transformation
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