Team:HokkaidoU Japan/Notebook/August31

From 2010.igem.org

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=pUC119のゲル抽＆Ligation&Transformation=
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* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
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* ゲル抽出(220 mg)
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* 50 uL（500 ng/uL）になったとする
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===Ligation===
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{|style="text-align:center;" width="500px"
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|-
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|style="border-right:1px solid #000; border-bottom:1px solid #000; width:100px"|
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|style="border-bottom:1px solid #000;"|なし
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|style="border-bottom:1px solid #000;"|I
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|style="border-bottom:1px solid #000;"|M
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|-
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|style="border-right:1px solid #000;"|DNA
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|2 uL
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|2 uL
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|2 uL
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|-
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|style="border-right:1px solid #000;"|ligation solution I
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|－
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|2 uL
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|－
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|-
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|style="border-right:1px solid #000;"|Mighty Mix
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|－
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|－
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|2 uL
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|-
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|style="border-right:1px solid #000;"|T4 ligase
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|－
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|0.4 uL
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|0.4 uL
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|-
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|style="border-right:1px solid #000; border-top:1px solid #000;"|Total
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|style="border-top:1px solid #000;"|2 uL
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|style="border-top:1px solid #000;"|4.4 uL
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|style="border-top:1px solid #000;"|4.4 uL
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|}
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→Transformation

Revision as of 12:42, 18 September 2010

pUC119のゲル抽＆Ligation&Transformation

  • pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え，３レーンに流した
  • ゲル抽出(220 mg)
  • 50 uL（500 ng/uL）になったとする

Ligation

なし I M
DNA 2 uL 2 uL 2 uL
ligation solution I － 2 uL －
Mighty Mix － － 2 uL
T4 ligase － 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
→Transformation
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