http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&feed=atom&action=history
Team:HokkaidoU Japan/Notebook/August30 - Revision history
2024-03-28T19:09:27Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=176449&oldid=prev
Sho N at 07:59, 27 October 2010
2010-10-27T07:59:00Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Electrophoresed 2 uL for confirmation</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Electrophoresed 2 uL for confirmation</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* There were no bands, forgot to add DNA <del class="diffchange diffchange-inline">:(</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* There were no bands, forgot to add DNA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Reused the remaining 18 uL of digestion solution by adding 1 uL of ADW and 1uL of DNA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Reused the remaining 18 uL of digestion solution by adding 1 uL of ADW and 1uL of DNA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-right:1px solid #996; border-bottom:1px solid #996;"| </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-right:1px solid #996; border-bottom:1px solid #996;"| </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #996; border-bottom:1px solid #996;"|RBS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #996; border-bottom:1px solid #996;"|RBS</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #996; border-bottom:1px solid #996;"|<del class="diffchange diffchange-inline">dT</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #996; border-bottom:1px solid #996;"|<ins class="diffchange diffchange-inline">double terminator</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style="border-bottom:1px solid #996;"|GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|colspan="4" style="border-bottom:1px solid #996;"|GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
</table>
Sho N
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=176427&oldid=prev
Sho N at 07:57, 27 October 2010
2010-10-27T07:57:05Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 07:57, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png <del class="diffchange diffchange-inline">Lambda</del>/''Hin''dIII, EcoR I](4 uL used)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png <ins class="diffchange diffchange-inline">λ</ins>/''Hin''dIII, EcoR I](4 uL used)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|3</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|3</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Incubated at 37C for 60 min</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Electrophoresis===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Electrophoresis===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Markers used [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png <del class="diffchange diffchange-inline">Lambda</del>/''Hin''dIII, EcoR I] and 50bp ladder</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Markers used [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png <ins class="diffchange diffchange-inline">λ</ins>/''Hin''dIII, EcoR I] and 50bp ladder</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Was obvious that parts were cut as intended</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Was obvious that parts were cut as intended</div></td></tr>
</table>
Sho N
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=176415&oldid=prev
Sho N at 07:56, 27 October 2010
2010-10-27T07:56:11Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 07:56, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|Pst I</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|Pst I</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|E, P</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|E, P</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|<del class="diffchange diffchange-inline">古いEcoR </del>I</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-bottom:1px;"|<ins class="diffchange diffchange-inline">Old EcoR </ins>I</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|DNA solution</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|DNA solution</div></td></tr>
</table>
Sho N
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=80177&oldid=prev
Laurynas: /* Digestion of pUC119 by EcoR I, Pst I */
2010-09-27T17:05:42Z
<p><span class="autocomment">Digestion of pUC119 by EcoR I, Pst I</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:05, 27 September 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-top:1px solid #996;"|20 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-top:1px solid #996;"|20 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830a.jpg|200px|right|thumb|]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830a.jpg|200px|right|thumb|<ins class="diffchange diffchange-inline">Electrophoresis of digestion products</ins>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">→同様にインキュベート&電気泳動</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">→Incubated at 37C for 60 min</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===<del class="diffchange diffchange-inline">電気泳動</del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===<ins class="diffchange diffchange-inline">Electrophoresis</ins>===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">マーカーはλ</del>/''Hin''<del class="diffchange diffchange-inline">d III</del>, <del class="diffchange diffchange-inline">EcoR Iと50bp のラダーマーカー</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Markers used [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png Lambda</ins>/''Hin''<ins class="diffchange diffchange-inline">dIII</ins>,<ins class="diffchange diffchange-inline"> EcoR I] and 50bp ladder</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* ほかは上の表の通り</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Was obvious that parts were cut as intended</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">ちゃんと切れてる</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>
Laurynas
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=80175&oldid=prev
Laurynas: /* ラオリプライマーで作ったパーツも同様にdigestion */
2010-09-27T17:02:01Z
<p><span class="autocomment">ラオリプライマーで作ったパーツも同様にdigestion</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:02, 27 September 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Because plasmid became linear it's was slower than super-coiled one's</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Because plasmid became linear it's was slower than super-coiled one's</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">ラオリプライマーで作ったパーツも同様にdigestion</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Digestion of parts PCRed using digestion visualization primers</ins>==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|style="text-align:center;" class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|style="text-align:center;" class="protocol"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
</table>
Laurynas
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=79921&oldid=prev
Laurynas: /* pUC119のEcoR I, Pst I digestion */
2010-09-27T13:28:40Z
<p><span class="autocomment">pUC119のEcoR I, Pst I digestion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August27|August 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August31|August 31]]</div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August27|August 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August31|August 31]]</div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<del class="diffchange diffchange-inline">pUC119のEcoR </del>I, Pst I <del class="diffchange diffchange-inline">digestion</del>=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=<ins class="diffchange diffchange-inline">Digestion of pUC119 by EcoR </ins>I, Pst I=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border="1" style="text-align:center;" class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border="1" style="text-align:center;" class="protocol"</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Electrophoresed 2 uL for confirmation</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→Electrophoresed 2 uL for confirmation</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* There were no bands, forgot to add DNA :(</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* There were no bands, forgot to add DNA :(</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">残った18 uLにDW </del>1 <del class="diffchange diffchange-inline">uLとDNAを加えて</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Reused the remaining 18 uL of digestion solution by adding </ins>1 <ins class="diffchange diffchange-inline">uL of ADW and 1uL of DNA</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">→37℃, </del>60 min<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">→Incubated at 37C for </ins>60 min<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">→2 uLを電気泳動で確認(</del>+ 0.4 uL 6x SB)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">→Electrophoresed 2 uL of each solution(</ins>+ 0.4 uL 6x SB)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Electrophoresis===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Electrophoresis===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|<ins class="diffchange diffchange-inline">Electrophoresis of digestion products</ins>]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''Lane'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''Lane'''</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 77:</td>
<td colspan="2" class="diff-lineno">Line 78:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">λ</del>/''Hin''<del class="diffchange diffchange-inline">d III</del>, <del class="diffchange diffchange-inline">EcoR I(4 uL使用)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png Lambda</ins>/''Hin''<ins class="diffchange diffchange-inline">dIII</ins>,<ins class="diffchange diffchange-inline"> EcoR I](4 uL used)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|3</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|3</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">切ってない</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">Undigested</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 92:</td>
<td colspan="2" class="diff-lineno">Line 93:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|7</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|7</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">古いEcoR </del>I</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">EcoR </ins>I <ins class="diffchange diffchange-inline">(used old enzyme to check it's activity)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">レーン3ではモノマー,ダイマー,トライマーなどのプラスミドが見られる.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">In lane 3 monomers, dimers and trimers of plasmid were visible .</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">レーン4~7ではこれらが切れているのが分かる</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">From lanes 4 through 7 it's visible that DNA digestion wasn't satisfactory</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** <del class="diffchange diffchange-inline">リニアになったので,モノマーの環状DNA(超コイル)より流れにくい</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** <ins class="diffchange diffchange-inline">Because plasmid became linear it's was slower than super-coiled one's</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==ラオリプライマーで作ったパーツも同様にdigestion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==ラオリプライマーで作ったパーツも同様にdigestion==</div></td></tr>
</table>
Laurynas
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=77179&oldid=prev
Laurynas: /* pUC119のEcoR I, Pst I digestion */
2010-09-23T08:50:41Z
<p><span class="autocomment">pUC119のEcoR I, Pst I digestion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:50, 23 September 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=pUC119のEcoR I, Pst I digestion=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=pUC119のEcoR I, Pst I digestion=</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border="1" style="text-align:center;" class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border="1" style="text-align:center;" class="protocol"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 60:</td>
<td colspan="2" class="diff-lineno">Line 61:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||'''20 uL'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>||'''20 uL'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">→37℃, </del>60 min<del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">→2 uLを電気泳動で確認</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">→Incubated at 37C for </ins>60 min</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">バカ橋さんがDNAを入れ忘れたためやりなおし</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">→Electrophoresed 2 uL for confirmation</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* 残った18 <del class="diffchange diffchange-inline">uLにDW1 </del>uLとDNAを加えて</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">There were no bands, forgot to add DNA :(</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* 残った18 <ins class="diffchange diffchange-inline">uLにDW 1 </ins>uLとDNAを加えて</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→37℃, 60 min<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→37℃, 60 min<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→2 uLを電気泳動で確認(+ 0.4 uL 6x SB)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>→2 uLを電気泳動で確認(+ 0.4 uL 6x SB)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===<del class="diffchange diffchange-inline">電気泳動</del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===<ins class="diffchange diffchange-inline">Electrophoresis</ins>===</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td></tr>
</table>
Laurynas
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=74922&oldid=prev
Sprkata: /* ラオリプライマーで作ったパーツも同様にdigestion */
2010-09-20T07:06:02Z
<p><span class="autocomment">ラオリプライマーで作ったパーツも同様にdigestion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:06, 20 September 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 97:</td>
<td colspan="2" class="diff-lineno">Line 97:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|style="text-align:center;" class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|style="text-align:center;" class="protocol"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>; border-bottom:1px solid #<del class="diffchange diffchange-inline">000</del>;"| </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-bottom:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"| </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>; border-bottom:1px solid #<del class="diffchange diffchange-inline">000</del>;"|RBS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-bottom:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|RBS</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>; border-bottom:1px solid #<del class="diffchange diffchange-inline">000</del>;"|dT</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-bottom:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|dT</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|colspan="4" style="border-bottom:1px solid #<del class="diffchange diffchange-inline">000</del>;"|GFP</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|colspan="4" style="border-bottom:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|DNA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|DNA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 116:</td>
<td colspan="2" class="diff-lineno">Line 116:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|DW</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|DW</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|13 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|13 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|13 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|13 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|17 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|14 uL</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 130:</td>
<td colspan="2" class="diff-lineno">Line 130:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|13 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|13 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|10x M buffer</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|10x M buffer</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|2 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|2 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 144:</td>
<td colspan="2" class="diff-lineno">Line 144:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|0.1% BSA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|0.1% BSA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|2 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|2 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 158:</td>
<td colspan="2" class="diff-lineno">Line 158:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|EcoR I</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|EcoR I</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 172:</td>
<td colspan="2" class="diff-lineno">Line 172:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|Pst I</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|Pst I</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|1 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 186:</td>
<td colspan="2" class="diff-lineno">Line 186:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<del class="diffchange diffchange-inline">000</del>; border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|Total</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|Total</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>; border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>; border-right:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>; border-right:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<del class="diffchange diffchange-inline">000</del>;"|20 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #<ins class="diffchange diffchange-inline">996</ins>;"|20 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830a.jpg|200px|right|thumb|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830a.jpg|200px|right|thumb|]]</div></td></tr>
</table>
Sprkata
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=74889&oldid=prev
Sprkata: /* 電気泳動 */
2010-09-20T06:38:36Z
<p><span class="autocomment">電気泳動</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 06:38, 20 September 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:HokkaidoU Japan 20100830b.jpg|200px|right|thumb|]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="protocol"</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|'''Lane'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|'''DNA'''</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2</div></td></tr>
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Sprkata
http://2010.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/August30&diff=74253&oldid=prev
Sprkata: /* ラオリプライマーで作ったパーツも同様にdigestion */
2010-09-19T05:52:44Z
<p><span class="autocomment">ラオリプライマーで作ったパーツも同様にdigestion</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 05:52, 19 September 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-right:1px solid #000; border-top:1px solid #000;"|Total</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="border-right:1px solid #000; border-top:1px solid #000;"|Total</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000; border-right:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000; border-right:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000; border-right:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000; border-right:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<del class="diffchange diffchange-inline">2-</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|style="border-top:1px solid #000;"|<ins class="diffchange diffchange-inline">20 uL</ins></div></td></tr>
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