Team:HokkaidoU Japan/Notebook/August27


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Transfer 1-1A on pSB1A10 to pSB1C3

1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before 2.mixed solutions according to the table below

Reagent Amount
pSB1C3 5 uL
DW 75 uL
10x M Buffer 10 uL
1%BSA 10 uL
EcoRI 5 uL
PstI 5 uL
Total 100 uL
Reagent Amount
1-1A 8 uL
DW 6 uL
10x M Buffer 2 uL
1%BSA 2 uL
EcoRI 1 uL
PstI 1 uL
Total 20 uL