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Team:HokkaidoU Japan/Notebook/August27
From 2010.igem.org
(Difference between revisions)
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=Transfer 1-1A on pSB1A10 to pSB1C3= | =Transfer 1-1A on pSB1A10 to pSB1C3= | ||
1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before | 1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before | ||
| - | |||
| + | 2.mixed solutions according to the table below | ||
| + | <div style="float:left;"> | ||
{|style="text-align: center;" class="protocol" | {|style="text-align: center;" class="protocol" | ||
|- | |- | ||
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|style="border-top:1px solid #996"|'''100 uL''' | |style="border-top:1px solid #996"|'''100 uL''' | ||
|} | |} | ||
| + | </div> | ||
| + | <div style="float:left; margin-left:50px;"> | ||
{|style="text-align: center;" class="protocol" | {|style="text-align: center;" class="protocol" | ||
|- | |- | ||
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|style="border-top:1px solid #996"|'''20 uL''' | |style="border-top:1px solid #996"|'''20 uL''' | ||
|} | |} | ||
| + | </div> | ||
Revision as of 12:03, 13 October 2010
since 13 Jul 2010
since 1 Aug 2010
ラオリ英語添削お願い
Transfer 1-1A on pSB1A10 to pSB1C3
1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before
2.mixed solutions according to the table below
| Reagent | Amount |
|---|---|
| pSB1C3 | 5 uL |
| DW | 75 uL |
| 10x M Buffer | 10 uL |
| 1%BSA | 10 uL |
| EcoRI | 5 uL |
| PstI | 5 uL |
| Total | 100 uL |
| Reagent | Amount |
|---|---|
| 1-1A | 8 uL |
| DW | 6 uL |
| 10x M Buffer | 2 uL |
| 1%BSA | 2 uL |
| EcoRI | 1 uL |
| PstI | 1 uL |
| Total | 20 uL |
