Team:HokkaidoU Japan/Notebook/August27

(Difference between revisions)
 Revision as of 11:58, 13 October 2010 (view source)Sho N (Talk | contribs)← Older edit Revision as of 12:03, 13 October 2010 (view source)Sho N (Talk | contribs) Newer edit → Line 5: Line 5: =Transfer 1-1A on pSB1A10 to pSB1C3= =Transfer 1-1A on pSB1A10 to pSB1C3= 1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before 1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before - 2.mixed solutions according to the table below + 2.mixed solutions according to the table below +
{|style="text-align: center;" class="protocol" {|style="text-align: center;" class="protocol" |- |- Line 33: Line 34: |style="border-top:1px solid #996"|'''100 uL''' |style="border-top:1px solid #996"|'''100 uL''' |} |} +
+
{|style="text-align: center;" class="protocol" {|style="text-align: center;" class="protocol" |- |- Line 59: Line 62: |style="border-top:1px solid #996"|'''20 uL''' |style="border-top:1px solid #996"|'''20 uL''' |} |} +

ラオリ英語添削お願い

Transfer 1-1A on pSB1A10 to pSB1C3

1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before

2.mixed solutions according to the table below

Reagent Amount
pSB1C3 5 uL
DW 75 uL
10x M Buffer 10 uL
1%BSA 10 uL
EcoRI 5 uL
PstI 5 uL
Total 100 uL
Reagent Amount
1-1A 8 uL
DW 6 uL
10x M Buffer 2 uL
1%BSA 2 uL
EcoRI 1 uL
PstI 1 uL
Total 20 uL