Team:HokkaidoU Japan/Notebook/August26

From 2010.igem.org

Revision as of 10:15, 22 September 2010 by Laurynas (Talk | contribs)

Electrophoresis of pSB1C3 concentrated to 50 ug/uL

Electrophoresis of concentrated pSB1C3
  • Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
Lane DNA
Added too much of marker, mistake
Lambda/Hind III & EcoR I
pSB1C3 solution
pSB1C3 solution
  • IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
  • Only band visible was monomer(about 2000 bp)

pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過

電気泳動で確かめた残り,49 uLをMicrocon YM-10でろ過した.