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Team:HokkaidoU Japan/Notebook/August26
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(→pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過) |
(→Electrophoresis of pSB1C3 concentrated to 50 ug/uL) |
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| - | = | + | =Electrophoresis of pSB1C3 concentrated to 50 ug/uL= |
[[Image:HokkaidoU Japan 20100826a.jpg|200px|right|thumb|Electrophoresis of concentrated pSB1C3]] | [[Image:HokkaidoU Japan 20100826a.jpg|200px|right|thumb|Electrophoresis of concentrated pSB1C3]] | ||
Revision as of 13:21, 24 September 2010
since 13 Jul 2010
since 1 Aug 2010
Electrophoresis of pSB1C3 concentrated to 50 ug/uL
- Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
| Lane | DNA |
| １ | Added too much of marker, mistake |
| ２ | Lambda/Hind III & EcoR I |
| ３ | pSB1C3 solution |
| ４ | pSB1C3 solution |
- IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
- Only band visible was monomer（about 2000 bp）
