Team:HokkaidoU Japan/Notebook/August26

From 2010.igem.org

(Difference between revisions)
(→50 ug/uLに濃縮したpSB1C3の電気泳動)
(→pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過)
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* Only band visible was monomer（about 2000 bp）
* Only band visible was monomer（about 2000 bp）
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=pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過=
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=Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions=
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電気泳動で確かめた残り，49 uLをMicrocon YM-10でろ過した．
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Remaining amount from check via electrophoresis，namely 49 uL was filtrated with  Microcon YM-10

Revision as of 10:17, 22 September 2010

Notebook

Electrophoresis of pSB1C3 concentrated to 50 ug/uL

Electrophoresis of concentrated pSB1C3
  • Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
Lane DNA
１ Added too much of marker, mistake
２ Lambda/Hind III & EcoR I
３ pSB1C3 solution
４ pSB1C3 solution
  • IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
  • Only band visible was monomer（about 2000 bp）

Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions

Remaining amount from check via electrophoresis，namely 49 uL was filtrated with Microcon YM-10
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