Team:HokkaidoU Japan/Notebook/August26

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Latest revision as of 07:54, 27 October 2010

Notebook

Electrophoresis of pSB1C3 concentrated to 50 ug/uL

Electrophoresis of concentrated pSB1C3
  • Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed
Lane DNA
１ Added too much of marker, mistake
２ λ/Hind III & EcoR I
３ pSB1C3 solution
４ pSB1C3 solution
  • IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
  • Only band visible was monomer（about 2000 bp）

Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions

Remaining amount from check via electrophoresis，namely 49 uL was filtrated with Microcon YM-10
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