Revision as of 08:52, 22 September 2010

Check to see if digestion visualization Primers Work

Electrophoresis after restriction enzyme digestion‎

Promoter band in lane 6 wasn't visible

TAKARABIO's Ligation Mixture [I] and Ligation Mixture Mighty Mix [M]

After PCR and gel extracted pSB1C3 volume of either Ligation Mixture equal to pSB1C3 solution volume was added
Transformed
incubated in 200 uL of LB medium
100 uL of each solution was plated on mediums containing 34 ug/uL and 12 ug/uL of chloramphenicol

レーン３でバンドが見られなかった．

レーン７は薄いがバンドが確認できた

@@ Line 29: / Line 29: @@
 * This vector didn't have a primer annealing site for our new primers
-=Ligation Mixtureのチェック=
+=Check of Ligation Mixtures=
-Ligation Mixture「I」とLigation Mixture「M」について
+TAKARABIO's Ligation Mixture [I] and Ligation Mixture Mighty Mix [M]
-* PCRで増やしてゲル抽したpSB1C3 2 uLにLigation Mixture 4 uLをいれ，それぞれのTransformationした
+* After PCR and gel extracted pSB1C3 volume of either Ligation Mixture equal to pSB1C3 solution volume was added
-* LB 200 uLを加え，100 uLずつをChloramphenicol 34 ug/uLと 12 ug/uLの培地にまいた
+* Transformed
+* incubated in 200 uL of LB medium
+* 100 uL of each solution was plated on mediums containing 34 ug/uL and 12 ug/uL of chloramphenicol
 =いろいろ確認=