Revision as of 17:46, 21 September 2010

pSB1C3 Activity Check

Used 2 uL of from 100 uL of gel extracted DNA for liagation
- Namely added 2 uL of Ligation Solution to 2 uL of DNA
Incubated at 16C for 30min

Used 2 uL of from 100 uL of gel extracted DNA for Transformation

pSB1A3, pSB1C3, pSB1T3それぞれについて以下の反応系でPCRを行った

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-=pSB1C3の活性評価=
+=pSB1C3 Activity Check=
 ==EcoR I/Pst I Digestion==
 {| class="protocol"
 |'''Reagent'''
@@ Line 29: / Line 30: @@
 |}
-* 37℃，60 min
+* Incubated at 37C for 60 min
-* 15 uL 6x Sample Bufferを加え，６レーンに分けて泳動
+* Added 15 uL of 6x Sample Buffer and electrophoresed on six lanes
-* ゲル抽出
+* Extracted from gel
-===Ligationあり===
+===Ligated===
-ゲル抽して得た100 uL DNAのうち2 uLを使用してTransformation
+*Used 2 uL of from 100 uL of gel extracted DNA for liagation
+** Namely added 2 uL of Ligation Solution to 2 uL of DNA
+*Incubated at 16C for 30min
-===Ligationなし===
+===Ligation Negative Control===
-ゲル抽して得た100 uL DNAのうち2 uLに2 uL Ligation Solutionを加える<br>
+Used 2 uL of from 100 uL of gel extracted DNA for Transformation
-℃で30分おき，計4 uLをTransformation
 =VectorのPCRリベンジ=