Team:HokkaidoU Japan/Notebook/August2
From 2010.igem.org
(Difference between revisions)
(→Result of Electrophoresis) |
|||
Line 1: | Line 1: | ||
{{Template:HokkaidoU_Japan}} | {{Template:HokkaidoU_Japan}} | ||
- | <div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/July27|July 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/ | + | <div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/July27|July 27]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August10|August 10]]</div></div> |
==Restriction enzyme digestion== | ==Restriction enzyme digestion== |
Latest revision as of 06:57, 27 October 2010
Restriction enzyme digestion
Added 30 uL of TE into the DNA solution (purified via miniprep)
Digestion Menu
sample 1 | sample 2 | sample 3 | sample 4 | |
DNA solution | 1 uL | 1 uL | 1 uL | 1 uL |
10x H Buffer | - | 1 uL | 1 uL | 1 uL |
DW | 9 uL | 7.5 uL | 7.0 uL | 7.5 uL |
Xba1 | - | 0.5 uL | 0.5 uL | - |
Pst1 | - | - | 0.5 uL | 0.5 uL |
- incubated at 37C for 1 hrs
- add 2 uL of 6x sample buffer
- Electrophoresed
Result of Electrophoresis
- The insert lenght wants 1069 bp (plus RE recognition site, 49 bp)
- Plasmid backbone was 5263 bp
- Marker was λ/Hind III and pUC119/Hinf I