Team:HokkaidoU Japan/Notebook/August17
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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August16|August 16]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August18|August 18]]</div></div> | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August16|August 16]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August18|August 18]]</div></div> | ||
- | = | + | =Gel Extraction= |
- | == | + | ==Electrophoresis== |
- | [[Image:HokkaidoU Japan 20100817a.JPG|200px|right|thumb|]] | + | |
- | + | [[Image:HokkaidoU Japan 20100817a.JPG|200px|right|thumb|Electrophoresis After Restriction Enzyme Digestion]] | |
+ | |||
+ | 20 uL of DNA digested yesterday was electrophoresed with 4 ul of 6x SB. All acording to the table bellow. | ||
+ | |||
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- | |λ/''Hin''d III | + | |[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png λ/''Hin''d III] |
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- | === | + | ===→[[Team:HokkaidoU_Japan/Protocols|Gel Extraction]]=== |
- | + | [[Image:HokkaidoU Japan 20100817b.jpg|200px|right|thumb|Electrophoresis to measure consentration after gel extraction]] | |
- | [[Image:HokkaidoU Japan 20100817b.jpg|200px|right|thumb|]] | + | * After ectraction DNA was electrophoresed to check consentration |
- | * | + | * 2 uL of 6x SB was added to DNA solution of 10 uL |
- | * DNA solution 10 | + | |
{| class="protocol" | {| class="protocol" | ||
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- | |λ/''Hin''d III | + | |[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png λ/''Hin''d III] |
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- | * | + | * Band of RBS isn't visible |
- | + | ** This Might due to loss durring extraction | |
+ | →will try again tomorrow | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
- | =Chloramphenicol | + | =Making of Chloramphenicol LB Medium= |
- | + | Because we were planing to use pSB1C3 we made LB Medium with chloramphenicol | |
- | * LB-broth | + | * Added 12.5 g LB-broth and 7.5 g of Agar 7.5 g to 500 mL to autoclaved distiled water, autoclave |
- | * 500 | + | * Added 500 uL of Chloramphenicol (34 mg/mL) |
- | * | + | * Poured it to 25 plates, 20 mL per plate |
Latest revision as of 07:36, 27 October 2010
Gel Extraction
Electrophoresis
20 uL of DNA digested yesterday was electrophoresed with 4 ul of 6x SB. All acording to the table bellow.
Lane | DNA |
1 | Empty |
2 | λ/Hind III |
3 | Empty |
4 | pSB1C3/E, P |
5 | pSB1C3/E, P |
6 | Heat Shock Promotor/E, S |
7 | Heat Shock Promotor/E, S |
8 | RBS/X, P |
9 | RBS/X, P |
10 | RFP/E, S |
11 | RFP/E, S |
12 | double Terminator/E, S |
13 | double Terminator/E, S |
14 | Empty |
15 | λ/Hind III |
16 | Empty |
17 | Empty |
→Gel Extraction
- After ectraction DNA was electrophoresed to check consentration
- 2 uL of 6x SB was added to DNA solution of 10 uL
Lane | DNA |
1 | Empty |
2 | λ/Hind III |
3 | Vector |
4 | Heat shock promotor |
5 | RBS |
6 | RFP |
7 | double terminator |
8 | Empty |
- Band of RBS isn't visible
- This Might due to loss durring extraction
→will try again tomorrow
Making of Chloramphenicol LB Medium
Because we were planing to use pSB1C3 we made LB Medium with chloramphenicol
- Added 12.5 g LB-broth and 7.5 g of Agar 7.5 g to 500 mL to autoclaved distiled water, autoclave
- Added 500 uL of Chloramphenicol (34 mg/mL)
- Poured it to 25 plates, 20 mL per plate