Team:Heidelberg/Project/miRNA Kit
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We further tested our kit using a gene that is an interesting candidate for gene therapy, human alpha-1-antitrypsin (haat) (ref, description). In this approach, we tagged haat, that we used as our GOI, with binding sites that we measured and characterized with our [https://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure] construct beforehand. This was a first potential therapeutic approach applying [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#ELISA ELISA] for measurements. | We further tested our kit using a gene that is an interesting candidate for gene therapy, human alpha-1-antitrypsin (haat) (ref, description). In this approach, we tagged haat, that we used as our GOI, with binding sites that we measured and characterized with our [https://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure] construct beforehand. This was a first potential therapeutic approach applying [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#ELISA ELISA] for measurements. | ||
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==Discussion== | ==Discussion== | ||
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==Methods== | ==Methods== | ||
The miTuner was [https://2010.igem.org/3A_Assembly assembled] out of different [https://2010.igem.org/Team:Heidelberg/Parts parts]. Cloning was done following [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Cloning standard protocols]. | The miTuner was [https://2010.igem.org/3A_Assembly assembled] out of different [https://2010.igem.org/Team:Heidelberg/Parts parts]. Cloning was done following [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Cloning standard protocols]. | ||
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Since the miTuner was constructed initially for [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Dual_Luciferase_Assay Dual Luciferase Assay] assay, this was the method of choice for tuning quantification. | Since the miTuner was constructed initially for [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Dual_Luciferase_Assay Dual Luciferase Assay] assay, this was the method of choice for tuning quantification. | ||
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==References== | ==References== | ||
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Bartel, D.P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281-297. | Bartel, D.P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281-297. | ||
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{{:Team:Heidelberg/Pagemiddle}} | {{:Team:Heidelberg/Pagemiddle}} | ||
Revision as of 21:16, 25 October 2010
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