Team:Heidelberg/Project/Attributions
From 2010.igem.org
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In the following we distinguish between students, advisors/instructors, external advisors, and service providers. | In the following we distinguish between students, advisors/instructors, external advisors, and service providers. | ||
- | == miMeasure | + | == miMeasure == |
<p>The design of the measurement standard construct for shRNA or miRNA silencing strengths has been supported by advisors of the team. This has been accompanied by the documentation on the measurement standard for synthetic promoters (Team Heidelberg 2009, [http://dspace.mit.edu/bitstream/handle/1721.1/49501/BBFRFC41.pdf?sequence=1 RFC 41]) and the prokaryotic promoter measurement kit (Jason Kelly, [http://bbf.openwetware.org/RFC.html#BBF_RFC_19:_Measuring_the_Activity_of_BioBrick.E2.84.A2_Promoters_Using_an_In_Vivo_Reference_Standard RFC 19]). The design in annotated sequence format of the final construct has been conducted by students. The basic construct has been synthesized by Geneart, and the following replacements of parts (in particular binding sites) within the construct have been cloned by students. This includes the design of cloning strategies.</p> | <p>The design of the measurement standard construct for shRNA or miRNA silencing strengths has been supported by advisors of the team. This has been accompanied by the documentation on the measurement standard for synthetic promoters (Team Heidelberg 2009, [http://dspace.mit.edu/bitstream/handle/1721.1/49501/BBFRFC41.pdf?sequence=1 RFC 41]) and the prokaryotic promoter measurement kit (Jason Kelly, [http://bbf.openwetware.org/RFC.html#BBF_RFC_19:_Measuring_the_Activity_of_BioBrick.E2.84.A2_Promoters_Using_an_In_Vivo_Reference_Standard RFC 19]). The design in annotated sequence format of the final construct has been conducted by students. The basic construct has been synthesized by Geneart, and the following replacements of parts (in particular binding sites) within the construct have been cloned by students. This includes the design of cloning strategies.</p> | ||
- | == miTuner | + | == miTuner == |
<p>The design of the tuning construct to silence target genes via shRNA has been supported by advisors. The final design in annotated sequence format has been conducted by students. Source DNA (e.g. luciferase gene, promoter sequences, terminators) has been provided by advisors. The DNA has been modified/reassembled via PCR/cloning by students.</p> | <p>The design of the tuning construct to silence target genes via shRNA has been supported by advisors. The final design in annotated sequence format has been conducted by students. Source DNA (e.g. luciferase gene, promoter sequences, terminators) has been provided by advisors. The DNA has been modified/reassembled via PCR/cloning by students.</p> | ||
- | == Design of shRNA sequences == | + | == Design of shRNA-like miRNA sequences == |
<p>The design of imperfect shRNA sequences to achieve various silencing strengths has been supported by advisors. The design in annotated sequence format and cloning of these sequences into the miMeasure construct has been done by students.</p> | <p>The design of imperfect shRNA sequences to achieve various silencing strengths has been supported by advisors. The design in annotated sequence format and cloning of these sequences into the miMeasure construct has been done by students.</p> | ||
Revision as of 08:31, 24 October 2010
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