Team:Heidelberg/Notebook/miRNA Kit

From 2010.igem.org

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(Synthetic miR Kit)
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= Synthetic miR Kit =
= Synthetic miR Kit =
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This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNAs and binding sites in an oligo and PCR based system.
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You do not know where to start? Why not [https://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner design your binding sites] first!
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After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasuremi Measure notebook] and see miMeasure in action on our [https://2010.igem.org/Team:Heidelberg/Project/miMeasure measurements] page!
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Revision as of 15:59, 24 October 2010

Synthetic miR Kit

This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNAs and binding sites in an oligo and PCR based system.

You do not know where to start? Why not design your binding sites first!

After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the Measure notebook and see miMeasure in action on our measurements page!


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