Team:Heidelberg/Notebook/miRNA Kit

From 2010.igem.org

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{{:Team:Heidelberg/Pagetop|note_mirna_kit}}
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= Synthetic miRNA Kit =
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{{:Team:Heidelberg/Side_Top}}
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This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNA-like miRNAs and referring binding sites using an oligo and PCR based system.
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You do not know where to start? Why don't you [https://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner design your binding sites] first!
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After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure mi Measure notebook] and see miMeasure in action on our [https://2010.igem.org/Team:Heidelberg/Project/miMeasure measurements] page!
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Now you would like to deliver you vector to a patient? Why not use a very specific [https://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling/ViroBytes shuttle]?
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{{:Team:Heidelberg/Pagemiddle}}
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{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#009be1; border: 1.5px solid #000000;"
{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#009be1; border: 1.5px solid #000000;"
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#23.2F08.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#24.2F08.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#25.2F08.2F2010 25]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#26.2F08.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#27.2F08.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#28.2F08.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#29.2F08.2F2010 29]'''
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#23.2F08.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#24.2F08.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#25.2F08.2F2010 25]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#26.2F08.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#27.2F08.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#28.2F08.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#29.2F08.2F2010 29]'''
|- style="background:#f2f2f2; color:#009be1"
|- style="background:#f2f2f2; color:#009be1"
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#30.2F08.2F2010 30]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/August#31.2F08.2F2010 31]'''||colspan="5"|
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|'''[[Igem2010/Main/synthetic_miR_Kit/August#30/08/2010|30]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#31/08/2010|31]]'''||colspan="5"|
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|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
|- style="background:#f2f2f2; color:#78b41e"
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|colspan="4"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#01.2F09.2F2010 1]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#02.2F09.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#03.2F09.2F2010 3]'''
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|colspan="4"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#01.2F10.2F2010 1]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#02.2F10.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#03.2F10.2F2010 3]'''
|- style="background:#f2f2f2; color:#f09600"
|- style="background:#f2f2f2; color:#f09600"
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#04.2F09.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#05.2F09.2F2010 5]'''
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#04.2F10.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#05.2F10.2F2010 5]'''
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#06.2F09.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#07.2F09.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#08.2F09.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#09.2F09.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#10.2F09.2F2010 10]'''
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#06.2F10.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#07.2F10.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#08.2F10.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#09.2F10.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#10.2F10.2F2010 10]'''
|- style="background:#f2f2f2; color:#f09600"
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#11.2F09.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#12.2F09.2F2010 12]'''|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#13.2F09.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#14.2F09.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#15.2F09.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#16.2F09.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#17.2F09.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#18.2F09.2F2010 18]'''
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#11.2F10.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#12.2F10.2F2010 12]'''|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#13.2F10.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#14.2F10.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#15.2F09.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#16.2F10.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#17.2F10.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#18.2F10.2F2010 18]'''
|- style="background:#f2f2f2; color:#f09600"
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#19.2F09.2F2010 19]'''||'''20'''||'''21'''||'''22'''||'''23'''||'''24'''||'''25'''
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|'''[https://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#19/10/2010 19]'''||'''20'''||'''21'''||'''22'''||'''23'''||'''24'''||'''25'''
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|'''26'''||'''27'''||'''28'''||'''29'''||'''30'''||colspan="3"|
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= Synthetic miRNA Kit =
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<center>
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[[Image:MiTuner p.png|400px| miTuner plasmid]]
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>/center>
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{{:Team:Heidelberg/Single_Bottom}}

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Synthetic miRNA Kit

miTuner plasmid

>/center>

Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit"