Team:Heidelberg/Notebook/ViroBytes/October

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Heidelberg/Template}} {{:Team:Heidelberg/Pagetop|note_virobytes}} =October= ==4/10/2010== *digest of 8 fragments with BsaI-HF @ 37°C for 1hr **6x2ul of F1-8 **3ul NEB4 10x **3u...)
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! colspan="7" style="background:#009be1;" | [http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/August<font color="white">August</font>]
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! colspan="7" style="background:#009be1;" | [http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September<font color="#ffecba">September</font>]
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|colspan="2"| ||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#01.2F09.2F2010 1]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#02.2F09.2F2010 2]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#03.2F09.2F2010 3]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#04.2F09.2F2010 4]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#05.2F09.2F2010 5]'''
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|- style="background:#f2f2f2; color:#78b41e"
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|'''2'''||'''3'''||'''4'''||'''5'''||'''6'''||'''7'''||'''8'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#06.2F09.2F2010 6]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#07.2F09.2F2010 7]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#08.2F09.2F2010 8]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#09.2F09.2F2010 9]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#10.2F09.2F2010 10]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#11.2F09.2F2010 11]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#12.2F09.2F2010 12]'''
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|'''9'''||'''10'''||'''11'''||'''12'''||'''13'''||'''14'''||'''15'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#13.2F09.2F2010 13]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#14.2F09.2F2010 14]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#15.2F09.2F2010 15]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#16.2F09.2F2010 16]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#17.2F09.2F2010 17]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#18.2F09.2F2010 18]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#19.2F09.2F2010 19]'''
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|- style="background:#f2f2f2; color:#78b41e"
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|'''16'''||'''17'''||'''18'''||'''19'''||'''20'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#22/08/2010|22]]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#20.2F09.2F2010 20]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#21.2F09.2F2010 21]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#22.2F09.2F2010 22]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#23.2F09.2F2010 23]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#24.2F09.2F2010 24]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#25.2F09.2F2010 25]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#26.2F09.2F2010 26]'''
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|- style="background:#f2f2f2; color:#78b41e"
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|'''[[Igem2010/Main/synthetic_miR_Kit/August#23/08/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|24]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#26/08/2010|26]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#27/08/2010|27]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#28/08/2010|28]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#29/08/2010|29]]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#27.2F09.2F2010 27]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#28.2F09.2F2010 28]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#29.2F09.2F2010 29]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#22.2F30.2F2010 30]'''||colspan="3"|
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|- style="background:#f2f2f2; color:#78b41e"
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|'''[[Igem2010/Main/synthetic_miR_Kit/August#30/08/2010|30]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#31/08/2010|31]]'''||colspan="5"|
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{| cellpadding="5" cellspacing="0" style="text-align: center; color:#f3d675; border: 3px solid #4e93a4;"
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! colspan="7" style="background:#4e93a4;" | [http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September<font color="#ffecba">September</font>]
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|- style="background:#4e93a4; color:white"
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|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
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|colspan="2"| ||'''[[Igem2010/Main/synthetic_miR_Kit/September#01/09/2010|1]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#02/09/2010|2]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#03/09/2010|3]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#04/09/2010|4]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#05/09/2010|5]]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/September#06/09/2010|6]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#07/09/2010|7]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#08/09/2010|8]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#09/09/2010|9]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#10/09/2010|10]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#11/09/2010|11]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#12/09/2010|12]]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/September#13/09/2010|13]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#14/09/2010|14]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#15/09/2010|15]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#16/09/2010|16]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#17/09/2010|17]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#18/09/2010|18]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#19/09/2010|19]]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/September#20/09/2010|20]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#21/09/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#22/09/2010|22]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#23/09/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#24/09/2010|24]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#25/09/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#26/09/2010|26]]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/September#27/09/2010|27]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#28/09/2010|28]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#29/09/2010|29]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#30/09/2010|30]]'''||colspan="3"|
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{| cellpadding="5" cellspacing="0" style="text-align: center; color:#f3d675; border: 3px solid #4e93a4;"
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{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#f09600; border: 1.5px solid #333333;"
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! colspan="7" style="background:#4e93a4;" | [http://2010.igem.org/Igem2010/Main/ViroBytes/October<font color="#ffecba">October</font>]
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! colspan="7" style="background:#78b41e;" | [http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October<font color="white">October</font>]
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|- style="background:#4e93a4; color:white"
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|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
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|- style="background:#f2f2f2; color:#78b41e"
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|colspan="4"| ||'''[[Igem2010/Main/synthetic_miR_Kit/October#01/10/2010|01]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#02/10/2010|02]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#03/10/2010|03]]'''
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|colspan="4"| ||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#01.2F09.2F2010 1]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#02.2F09.2F2010 2]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#03.2F09.2F2010 3]'''
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|- style="background:#f2f2f2; color:#78b41e"
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|'''[[Igem2010/Main/synthetic_miR_Kit/October#04/10/2010|04]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#05/10/2010|05]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#06/10/2010|06]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#07/10/2010|07]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#08/10/2010|08]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#09/10/2010|09]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#10/10/2010|10]]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#04.2F09.2F2010 4]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#05.2F09.2F2010 5]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#06.2F09.2F2010 6]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#07.2F09.2F2010 7]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#08.2F09.2F2010 8]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#09.2F09.2F2010 9]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#10.2F09.2F2010 10]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/October#11/10/2010|11]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#12/10/2010|12]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#13/10/2010|13]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#14/10/2010|14]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#15/10/2010|15]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#16/10/2010|16]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#17/10/2010|17]]'''
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|- style="background:#f2f2f2; color:#78b41e"
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#11.2F09.2F2010 11]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#12.2F09.2F2010 12]'''|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#13.2F09.2F2010 13]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#14.2F09.2F2010 14]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#15.2F09.2F2010 15]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#16.2F09.2F2010 16]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#17.2F09.2F2010 17]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#18.2F09.2F2010 18]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/October#18/10/2010|18]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#19/10/2010|19]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#20/10/2010|20]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#21/10/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#22/10/2010|22]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#23/10/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#24/10/2010|24]]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#19.2F09.2F2010 19]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#20.2F09.2F2010 20]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#21.2F09.2F2010 21]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#22.2F09.2F2010 22]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#23.2F09.2F2010 23]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#24.2F09.2F2010 24]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#25.2F09.2F2010 25]'''
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|'''[[Igem2010/Main/synthetic_miR_Kit/October#25/10/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#26/10/2010|26]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#27/10/2010|27]]'''||'''28'''||'''29'''||'''30'''||'''31'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#26.2F09.2F2010 26]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#27.2F09.2F2010 27]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#28.2F09.2F2010 28]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#29.2F09.2F2010 29]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#22.2F30.2F2010 30]'''||colspan="3"|
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{{:Team:Heidelberg/Bottom}}
{{:Team:Heidelberg/Bottom}}

Revision as of 17:01, 26 October 2010

October

4/10/2010

  • digest of 8 fragments with BsaI-HF @ 37°C for 1hr
    • 6x2ul of F1-8
    • 3ul NEB4 10x
    • 3ul BSA 10x
    • 11ul H2O
    • 1ul BsaI-HF
  • purification with Nucleotide Removal Kit
  • NanoDrop concentration check:


Fragment 1 Fragment 2 Fragment 3 Fragment 4 Fragment 5 Fragment 6 Fragment 7 Fragment 8
35.5 ng/ul 43.5 ng/ul 43.0 ng/ul 43.0 ng/ul 79.0 ng/ul 51.0 ng/ul 63.0 ng/ul 44.0 ng/ul


5/10/2010

  • assembly started with Quick ligase kit
  • ~200ng of fragment DNA per reaction
  • ligation time ~10min

September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
27282930
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October
MTWTFSS
123
45 678910
11131415161718
19202122232425
2627282930
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