Team:Heidelberg/Notebook/MiMeasure/October

From 2010.igem.org

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[https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Microscopy microscopy] measurements  
[https://2010.igem.org/Team:Heidelberg/Notebook/Methods#Microscopy microscopy] measurements  
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[[Image:M12-M22_HeLa_daten.jpg‎|thumb|500px|center|''' different binding sites for miRsAg''']]
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[[Image:M12-M22_HeLa_daten.jpg‎|thumb|500px|left|''' different binding sites for miRsAg''']]
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[[Image:MiMeasure_miR122.jpg‎‎|thumb|500px|center|''' different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid''']]
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[[Image:MiMeasure_miR122.jpg‎‎|thumb|500px|left|''' different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid''']]
[[Image:MiMeasure_miR122b.jpg‎‎|thumb|500px|center|''' different binding sites for miR122, Huh7 cells''']]
[[Image:MiMeasure_miR122b.jpg‎‎|thumb|500px|center|''' different binding sites for miR122, Huh7 cells''']]
Flow cytometry measurements
Flow cytometry measurements
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[[Image:Flow.jpg|thumb|500px|left|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']]
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[[Image:Flow.jpg|thumb|500px|center|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']]
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[[Image:HeLa_miR122.jpg‎‎|thumb|500px|left|]]
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[[Image:HeLa_miR122.jpg‎‎|thumb|500px|center|]]
[[Image:HeLa_miR122_FACS.jpg‎‎|thumb|500px|center|]]
[[Image:HeLa_miR122_FACS.jpg‎‎|thumb|500px|center|]]
[[Image:HeLa_miR122_microscopy.jpg‎‎|thumb|500px|center|]]
[[Image:HeLa_miR122_microscopy.jpg‎‎|thumb|500px|center|]]

Revision as of 03:05, 28 October 2010

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21/10/2010

seeding HeLa and Huh7 in 96-well plates

22/10/2010

Transfection

23/10/2010

seeding HeLa and Huh7 in 96-well plates

24/10/2010

HeLa cells were transfected with miMeasure constructs with GFP expression regulated by different imperfect binding sites for miRsAg. As controls we used miMeasure construct with perfect binding site and no binding site. Each miMeasure was cotransfected with pcDNA plasmid expressing synthetic miRsAg and pcDNA5 expressing synthetic shRNA3.


Transfection of HeLa and Huh7 cells with miMeasure with binding sites for miR122.

Huh7 were transfected with 50ng of miMeasure. HeLa cells were used as a control they were transfected with miMeasure constructs and miR122. Control without miR122 expression plasmid was made.

10/26/2010

microscopy measurements

different binding sites for miRsAg
different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid
different binding sites for miR122, Huh7 cells

Flow cytometry measurements

GFP/BFP ratio of single cells according to flow cytometry analysis
HeLa miR122.jpg
HeLa miR122 FACS.jpg
HeLa miR122 microscopy.jpg
HepG2 miR122 microscopy.jpg
HuH7 miR122b.jpg
SAg miR155 HeLa.jpg
Single cellmiRNA expression.jpg
Single cellmiRNA expression cells.jpg
HepG2.jpg
Huh7.jpg
Cell lines mir122.jpg
Hela miRNA122 ratio.jpg

10/27/2010

HuhvsHelaoverview.jpg
MiR122.jpg