Team:Heidelberg/Notebook/Methods
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Laura Nadine (Talk | contribs) (→Quantitative Realtime PCR) |
Laura Nadine (Talk | contribs) (→Quantitative Realtime PCR) |
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Thermocycler program was 10min at 95°C initial activation, followed by 40 cycles of 10s at 95°C and 20s at 60°C. | Thermocycler program was 10min at 95°C initial activation, followed by 40 cycles of 10s at 95°C and 20s at 60°C. | ||
- | To find out the concentration of viral genomes per ml (Y)the value of the X position on the standard curve has to be multiplied as follows: <font face="Symbol">Y</font> = <font face="Symbol">X</font> ×10² ×10² | + | To find out the concentration of viral genomes per ml (<font face="Symbol">Y</font>) the value of the X position on the standard curve has to be multiplied as follows: <font face="Symbol">Y</font> = <font face="Symbol">X</font> ×10² ×10² |
== Measurements == | == Measurements == |
Revision as of 17:09, 25 October 2010
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