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Team:Heidelberg/Notebook/BSDesign/October
From 2010.igem.org
Revision as of 19:18, 27 October 2010 by
Leaflocke
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Home
Team
Overview
Students
Instructors
Advisors
Institutes
Gallery
miDANCE
Acknowledgements
Project
Abstract
Introduction
Synthetic miRNA Kit
Capsid Shuffling
Homology Based
ViroBytes
miMeasure
in vivo
Study
Summary & Outlook
References
Contributions
Parts
Parts
Characterization
Modeling
Introduction
miBEAT
miRockdown
miBS designer
mUTING
Descriptions
Notebook
Synthetic miRNA Kit
Capsid Shuffling
Homology Based
ViroBytes
miMeasure
Binding Site Design
in vivo
Study
Modeling
Materials
Methods
Psychological Survey
Philosophical Reflection
Biosafety
Human Practices
Psychological Survey
Philosophical Reflection
Sponsors
Binding Site Design - October
02/11/2010
digestion in 50 µl of
4x pSB1C3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
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