Team:Heidelberg/Notebook/BSDesign/October

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(→Binding Site Design - October)
(→10/02/2010)
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**4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
**4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
 +
== 10/05/2010 ==
 +
<br />
 +
*1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI
 +
1= 0S
 +
2= 0L
 +
3= 10S
 +
4= 10L
 +
20 Miniprep:
 +
{| class="wikitable sortable" border="0" align="center" style="text-align: left"
 +
|-bgcolor=#009be1
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|+ align="top, left"|'''Table 1: 20 Minis'''
 +
|Number||binding site against miRNA x||Position
 +
|-
 +
|1||221||3.5
 +
|-
 +
|2||221||1.4
 +
|-
 +
|3||1179||3.1
 +
|-
 +
|4||4286||2.6
 +
|-
 +
|5||4286||4.1
 +
|-
 +
|6||4286||3.4
 +
|-
 +
|7||1179||4.4
 +
|-
 +
|8||1179|3.5
 +
|-
 +
|9||1179||1.9
 +
|-
 +
|10||221||4.6
 +
|-
 +
|11||221||1.5
 +
|-
 +
|12||4286||3.5
 +
|-
 +
|13||221||4.8
 +
|-
 +
|14||221||2.3
 +
|-
 +
|15||4286||2.2
 +
|-
 +
|16||4286||1.3
 +
|-
 +
|17||1179||2.5
 +
|-
 +
|18||1179||2.4
 +
|-
 +
|19||1179||1.5
 +
|-
 +
|20||1179||4.8
 +
|-
----
----
{{:Team:Heidelberg/Single_Bottom}}
{{:Team:Heidelberg/Single_Bottom}}

Revision as of 19:41, 27 October 2010
 


Binding Site Design - October

10/02/2010


  • digestion in 50 µl of
    • 4x pSB1C3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
    • 4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)

10/05/2010


  • 1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI

1= 0S 2= 0L 3= 10S 4= 10L

20 Miniprep:

Table 1: 20 Minis
Numberbinding site against miRNA xPosition
12213.5
22211.4
311793.1
442862.6
542864.1
642863.4
711794.4
83.5
911791.9
102214.6
112211.5
1242863.5
132214.8
142212.3
1542862.2
1642861.3
1711792.5
1811792.4
1911791.5
2011794.8

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