Team:Heidelberg/Notebook/BSDesign/October

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(Binding Site Design - October)
(02/11/2010)
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== 02/11/2010 ==
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== 11/02/2010 ==
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*digestion in 50 µl of  
*digestion in 50 µl of  

Revision as of 19:19, 27 October 2010

 


Binding Site Design - October

11/02/2010


  • digestion in 50 µl of
    • 4x pSB1C3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
    • 4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)