Team:Heidelberg/Notebook/BSDesign

From 2010.igem.org

Revision as of 23:38, 27 October 2010 by Kleinsorg (Talk | contribs)

July
MTWTFSS
1234
567891011
12131415161718
19202122232425
262728293031

-


August
MTWTFSS
1
2345678
9101112131415
16171819202122
23242526272829
3031


September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
27282930

-


October
MTWTFSS
123
45678910
11121314151617
18192021222324
25262728293031

-

 


Binding Site Design

Introduction

To create binding site (BS) patterns for micro RNAs (miRNAs) , we used the random assembly PCR (raPCR) – method from iGEM2009-Heidelberg team (see here) and adopted it to our purposes. The differences:

  • Sequences from 100 to 400 base pairs are requested.
  • Oligos span over a whole binding site for a certain miRNA and shuffling occurs on the level of pattern creation.

Several points need to be considered for setting up miRNA-binding site (miRBS) patterns:

  • the right distance after the stop codon for efficient (or non-efficient) BS recognition
  • distance and sequence between miRBS (the spacer)


See on our Notebook pages how we created binding site patterns.

The adopted method for BS-patterns can be found on our methods page.

On the Parts-Page you can find standardized BS-patterns for hsa-mir-122 and has-mir-221, containing at least 2 binding sites.