Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 87: Line 87:
<div class="title">
<div class="title">
Modify AU content</div>
Modify AU content</div>
-
<table>
+
<table style="line-height:2;">
<tr>
<tr>
<td width=130px>
<td width=130px>
Line 102: Line 102:
</table>
</table>
</td>
</td>
-
<td style="vertical-align:top;">
+
<td>
<div class="title">Sticky ends for integration into plasmid</div>
<div class="title">Sticky ends for integration into plasmid</div>
<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
Line 128: Line 128:
{{:Team:Heidelberg/Pagemiddle}}
{{:Team:Heidelberg/Pagemiddle}}
<html>
<html>
-
<div class="wrapper"><br>
+
<div class="wrapper">
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<br><br>
<br><br>

Revision as of 09:26, 26 October 2010

BS designer for miRNA
Construction of microRNA binding sites


Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position
Supplementary region
Customized MATCH positions
Modify AU content
A in position -1
A in position 1
A in position 9
A in position 0
A in position 8
A in position 10
Sticky ends for integration into plasmid
Customized:
5'  
3'  



Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2